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How do N.A. and magnification relate to the brightness of an image?
The higher the N.A. value, for a given magnification, the brighter the image. A higher magnification reduces the brightness of an image.
This number is the numerical aperture of the objective, a measure of the light-gathering capacity of the objective. The higher the numerical aperture, other things being equal, the better the objective is able to separate the details of the specimen in forming an image; also the brighter the image. Higher N.A. objectives are usually more expensive. The higher the numerical aperture of the objective, the shallower the depth of field (see definition of depth of field).
Ultimo aggiornamento: 11 Jun 2019
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Categorie F.A.Q