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Can a N-PLAN or W-PLAN objective be useable in reflected light fluorescence?
The answer is a provisional yes. Planachromats may serve satisfactorily for blue or green excitation waves lengths. However, the planachromat's glass elements may themselves fluoresce in excitation in the near ultraviolet. Also, planachromats, for their respective magnifications, have lower N.A.'s than do PLAN F or PLAN APO thus the images may be not as bright.
Ultimo aggiornamento: 11 Jun 2019
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Categorie F.A.Q